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KMID : 0620919960280010011
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Experimental & Molecular Medicine
1996 Volume.28 No. 1 p.11 ~ p.16
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Effect of protein kinase C, calcium and CAMP on the expression of proto-oncogenes, pRb and Cell proliferation
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Moon Soon-Ok
Kim Dae-Ho
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Abstract
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The second messengers induced by growth factors are thought to have important roles in transducing the growth factor signals to nuclear events and cell proliferation. Various second messengers stimulate expression of proto-oncogenes such as c-fos and c-myc which are believed to have an important role in cell proliferation. To determine the causal relationship between these events we studied the effects of 12-0-tetradecanoyl phorbol-13-acetate, A23187 and dibutylyl cAMP plus 3-isobutyl-1-methylxanthine on the cell proliferation and the expression of c-fos and c-myc by using serum-deprived quiescent NIH 3T3 cells. Although A23187 stimulated the expression of these two proto-oncogenes most strongly, it stimulated cell proliferation only weakly, indicating that the expression of these proto-oncogenes are not sufficient for proliferation. Also we investgated the involvement of expression or phosphorylation of pRb in expression of c-fos and c-myc. Western blot analysis showed no significant changes in pRb expression or phosphorylation, indicating that second messengers induced expression of these proto-oncogene not through the activation of a transcription factor E2F which has been suggested to function in serum induced stimulation of these genes.
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KEYWORD
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signal transduction, second messenger systems, proto-oncogenes, cell division
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